Coding
Part:BBa_K2719010:Design
Designed by: Juan Carlos Rueda Silva & Arantxa Karam Coppola Group: iGEM18_TecCEM (2018-10-10)
Leptin coding device with blue chromoprotein
Here the part is composed by tow promoters, one for Blue Chromoprotein cassette and the other one for leptin (T7-lac induced by IPTG). Also, it has the coding sequences for blue chromoprotein and leptin and double terminator for both cases. The purpose of Blue chromoprotein was to simplify the selection of colonies. (Figure 1)
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Figure 1. Diagram of BBa_K2719010, leptin expression device
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Leptin codiNg device with blue chromoprotein
Source
Leptin codiNg device with blue chromoprotein